Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo

0384274 - ÚŽFG 2013 RIV US eng J - Článek v odborném periodiku
Šolc, Petr - Baran, Vladimír - Mayer, Alexandra - Böhmová, Tereza - Panenková, Gabriela - Šašková, Adéla - Schultz, R. M. - Motlík, Jan
Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo.
Biology of Reproduction. Roč. 87, č. 4 (2012), s. 1-12. ISSN 0006-3363. E-ISSN 1529-7268
Grant CEP: GA MŠMT ME08030; GA MŠMT LH12057; GA ČR(CZ) GPP301/11/P081
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: γ-tubulin * AURKA * CDC25B
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.027, rok: 2012

Aurora kinase A (AURKA) is an important mitotic kinase involved in the G2/M transition, centrosome maturation and separation, and spindle formation in somatic cells. We used transgenic models that specifically overexpress in mouse oocytes either wild-type (WT-AURKA) or a catalytically inactive (kinasedead) (KD-AURKA) AURKA to gain new insights regarding the role of AURKA during oocyte maturation. AURKA activation occurs shortly after hCG administration that initiates maturation in vivo. Although AURKA activity is increased in WT-AURKA oocytes, resumption of meiosis is not observed in the absence of hCG administration. Control oocytes contain one to three microtubule organizing centers (MTOCs; centrosome equivalent) at prophase I. At the time of germinal vesicle breakdown (GVBD), the first visible marker of resumption of meiosis, the MTOC number increases. In WT-AURKA oocytes, the increase in MTOC number occurs prematurely but transiently without GVBD, whereas the increase in MTOC number does not occur in control and KD-AURKA oocytes. AURKA activation is biphasic with the initial activation not requiring CDC25BCDK1 activity, whereas full activation, which is essential for the increase in MTOCs number, depends on CDK1 activity. AURKA activity also influences spindle length and regulates, independent of its protein kinase activity, the amount of MTOC associated with gamma-tubulin. Both WT-AURKA and KDAURKA transgenic mice have normal fertility during first 6 mo of life. These results suggest that although AURKA is not a trigger kinase for G2/M transition in mouse oocytes, it regulates MTOC number and spindle length, and,independent of its protein kinase activity, gamma-tubulin recruitment to MTOCs.
Trvalý link: http://hdl.handle.net/11104/0213976