Počet záznamů: 1  

Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration

  1. 1.
    0379290 - UEK-B 2013 RIV SK eng J - Článek v odborném periodiku
    Hrstka, M. - Urban, Otmar - Babák, L.
    Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration.
    Chemical Papers. Roč. 66, č. 9 (2012), s. 836-841. ISSN 0366-6352
    Grant CEP: GA AV ČR IAA600870701; GA MŠk(CZ) LM2010007; GA MŠk(CZ) ED1.1.00/02.0073
    Výzkumný záměr: CEZ:AV0Z60870520
    Klíčová slova: Rubisco content * Rubisco activity * seasonal changes * elevated CO2 concentrations * Fagus sylvatica * Picea abies
    Kód oboru RIV: EH - Ekologie - společenstva
    Impakt faktor: 0.879, rok: 2012

    We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2.
    Trvalý link: http://hdl.handle.net/11104/0007110