Počet záznamů: 1  

Differential response of Drosophila cell lines to extracellular adenosine

  1. 1.
    0376113 - BC 2013 RIV GB eng J - Článek v odborném periodiku
    Fleischmannová, J. - Kučerová, Lucie - Šandová, Kateřina - Steinbauerová, Veronika - Brož, Václav - Šimek, Petr - Žurovec, Michal
    Differential response of Drosophila cell lines to extracellular adenosine.
    Insect Biochemistry and Molecular Biology. Roč. 42, č. 5 (2012), s. 321-331. ISSN 0965-1748
    Grant CEP: GA MŠk(CZ) LC06077
    Grant ostatní:AV ČR(CZ) KJB501410801; European Community´s Seventh Framwork Programme (FP7/2007-2013)(CZ) 229518
    Výzkumný záměr: CEZ:AV0Z50070508
    Institucionální podpora: RVO:60077344
    Klíčová slova: adenosine recycling * nucleoside transport * Mbn2
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 3.234, rok: 2012
    http://www.sciencedirect.com/science/article/pii/S0965174812000033

    Adenosine (Ado) is a crucial metabolite that influences proliferation of several vertebrate and invertebrate cells. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8ţ) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by ATP production as a part of Ado salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.
    Trvalý link: http://hdl.handle.net/11104/0208606