Počet záznamů: 1  

Tools for genetic manipulations in Corynebacterium glutamicum and their applications

  1. 1. 0370930 - MBU-M 2012 RIV DE eng J - Článek v odborném periodiku
    Nešvera, Jan - Pátek, Miroslav
    Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
    Applied Microbiology and Biotechnology. Roč. 90, č. 5 (2011), s. 1641-1654. ISSN 0175-7598
    Grant CEP: GA ČR GC204/09/J015
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Corynebacterium glutamicum * Plasmid vectors * Promoters
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.425, rok: 2011

    Corynebacterium glutamicum is an important industrial producer of various amino acids with great potential for the production of other metabolites. The complete genome sequences of two C. glutamicum strains were determined and the use of genome-based approaches (transcriptomics, proteomics, metabolomics, and fluxomics) provided large amounts of data on the metabolism of this bacterium and its regulation. Many tools for genetic manipulations in C. glutamicum have been developed and used for the analysis of gene functions as well as for the construction and improvement of production strains. These tools include various types of plasmid vectors (cloning, promoter-probe, and expression vectors), DNA transfer methods, cloning heterologous genes, introducing protein secretion systems and gene replacement and genome rearrangement methods
    Trvalý link: http://hdl.handle.net/11104/0204602