Počet záznamů: 1  

Efficient biolistic transformation of the moss Physcomitrella patens

  1. 1. 0370792 - UEB-Q 2012 RIV NL eng J - Článek v odborném periodiku
    Šmídková, Markéta - Holá, M. - Angelis, Karel
    Efficient biolistic transformation of the moss Physcomitrella patens.
    Biologia Plantarum. Roč. 54, č. 4 (2010), s. 777-780 ISSN 0006-3134
    Grant CEP: GA ČR GA521/04/0971; GA AV ČR IBS5038304; GA MŠk 1M0505; GA MŠk(CZ) LC06004
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Helios biolistic gun * moss protonemal tissue * particle size
    Kód oboru RIV: EI - Biotechnologie a bionika
    Impakt faktor: 1.582, rok: 2010

    High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. The bombardment of tissue with 1 mu m gold particles yielded between 20 and 40 stable transformants per 1 mu g of DNA. Transformation with circular plasmids generates higher frequencies of random transgene integration, whereas linear plasmids are more efficient in generating gene-targeted insertions.
    Trvalý link: http://hdl.handle.net/11104/0204485