Počet záznamů: 1  

Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing

  1. 1.
    0370431 - BFU-R 2012 RIV US eng J - Článek v odborném periodiku
    Matyášek, Roman - Fulneček, Jaroslav - Leitch, A.R. - Kovařík, Aleš
    Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing.
    New Phytologist. Roč. 192, č. 3 (2011), s. 747-759. ISSN 0028-646X
    Grant CEP: GA ČR(CZ) GA206/09/1751; GA ČR(CZ) GAP501/10/0208; GA MŠk(CZ) LC06004
    Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Klíčová slova: DNA-curvature * subtelomeric satellite repeats * allopolyploidy
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 6.645, rok: 2011

    In allotetraploid Nicotiana arentsii, we examined the fate of homologous subtelomeric satellites NUNSSP and NWISSP inherited from diploid progenitors N. undulata and N. wigandioides, respectively. While the NUNSSP monomers were highly homogeneous in nucleotide sequences, the NWISSP monomers formed two separate clades. Likewise, the genomic NUNSSP monomers showed less DNA conformation heterogeneity than NWISSP monomers, with distinct conformations. Sequence, structural and chromosomal features of the parental satellites were faithfully inherited by N. arentsii. Our study revealed that inter-genomic homogenisation of subtelomeric satellite repeats does not occur in N. arentsii allotetraploid. We propose that the sequence and structural divergence of subtelomeric satellites may render allopolyploid chromosomes less vulnerable to intergenomic exchanges.
    Trvalý link: http://hdl.handle.net/11104/0204234