Počet záznamů: 1
Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing
- 1.0370431 - BFÚ 2012 RIV US eng J - Článek v odborném periodiku
Matyášek, Roman - Fulneček, Jaroslav - Leitch, A.R. - Kovařík, Aleš
Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing.
New Phytologist. Roč. 192, č. 3 (2011), s. 747-759. ISSN 0028-646X. E-ISSN 1469-8137
Grant CEP: GA ČR(CZ) GA206/09/1751; GA ČR(CZ) GAP501/10/0208; GA MŠMT(CZ) LC06004
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: DNA-curvature * subtelomeric satellite repeats * allopolyploidy
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 6.645, rok: 2011
In allotetraploid Nicotiana arentsii, we examined the fate of homologous subtelomeric satellites NUNSSP and NWISSP inherited from diploid progenitors N. undulata and N. wigandioides, respectively. While the NUNSSP monomers were highly homogeneous in nucleotide sequences, the NWISSP monomers formed two separate clades. Likewise, the genomic NUNSSP monomers showed less DNA conformation heterogeneity than NWISSP monomers, with distinct conformations. Sequence, structural and chromosomal features of the parental satellites were faithfully inherited by N. arentsii. Our study revealed that inter-genomic homogenisation of subtelomeric satellite repeats does not occur in N. arentsii allotetraploid. We propose that the sequence and structural divergence of subtelomeric satellites may render allopolyploid chromosomes less vulnerable to intergenomic exchanges.
Trvalý link: http://hdl.handle.net/11104/0204234
Počet záznamů: 1