Počet záznamů: 1
Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers
- 1.0366150 - MBÚ 2012 RIV DE eng J - Článek v odborném periodiku
Marhol, Petr - Gažák, Radek - Bednář, P. - Křen, Vladimír
Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers.
Journal of Separation Science. Roč. 34, 16-17 (2011), s. 2206-2213. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA MŠMT(CZ) ME10027; GA ČR GAP207/10/0288
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: chromolith * kinetex * Core-shell
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 2.733, rok: 2011
Two chromatographic narrow-bore columns, a novel 2.6 µm particle-packed KinetexTM C18 core–shell (50x2.1mm id) and monolithic Chromolith® FastGradient RP-18e (50x2mm id), were evaluated for the analysis of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin under isocratic conditions. The main advantages of the core–shell column are markedly higher efficiency (hmin=2.8 versus 5.6 for silybin A) and better peak symmetry. The Kinetex column exhibits only a slight change in the height equivalent of the theoretical plate with a higher linear velocity of the mobile phase. The monolithic column shows notably higher selectivity in terms of selectivity factor (1.21 versus 1.12) in the analysis of critical-pair of diastereoisomers (silybin A and silybin B) and enables shorter run duration (approx. twofold) together with lower backpressure
Trvalý link: http://hdl.handle.net/11104/0201225
Počet záznamů: 1