Synthesis of biaryl-substituted fluorescent nucleosides and nucleoside triphosphates and their incorporation to DNA

0361534 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Riedl, Jan - Hocek, Michal
Synthesis of biaryl-substituted fluorescent nucleosides and nucleoside triphosphates and their incorporation to DNA.
Chemistry of Nucleic Acid Components. 15th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i., 2011 - (Hocek, M.), s. 233-235. Collection Symposium Series, 12. ISBN 978-80-86241-37-1.
[Chemistry of Nucleic Acid Components /15./. Český Krumlov (CZ), 05.06.2011-10.06.2011]
Grant CEP: GA MŠMT LC512; GA ČR GA203/09/0317
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: nucleotides * fluorescence * DNA
Kód oboru RIV: CC - Organická chemie

A series of modified nucleosides bearing the fluorescent substituted biaryl labels attached to nucleobase was prepared and their luminescent properties were evaluated. The modified nucleosides show divers fluorescence from 400 to 560 nm when excited at 340 nm in water depending on structural and substitution pattern. The corresponding biaryl-substituted nucleoside triphosphates (dNTPs) were prepared analogously and used for polymerase incorporation to DNA. Applications in hybridization probes were studied.
Trvalý link: http://hdl.handle.net/11104/0198829