0172432 - UEB-Q 20033020 RIV CA eng J - Článek v odborném periodiku
Doleželová, Marie - Valárik, Miroslav - Bartoš, Jan - Vrána, Jan - Čihalíková, Jarmila - Molnár-Láng, M. - Doležel, Jaroslav
Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry.
Genome. Roč. 46, - (2003), s. 893-905. ISSN 0831-2796. E-ISSN 1480-3321
Grant CEP: GA AV ČR KSK5052113; GA MZe QC1336
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Chromosome sorting * fluorescence in situ hybridization * repetitive DNA sequences
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.861, rok: 2003

Procedures for chromosome analysis and sorting using flow cytometry were developed for rye (Secale cereale L.). Chromosome 1R could be discriminated on a flow caryotype. Remaining rye chromosomes (2R-7R) could be discriminated and sorted from individual wheat-rye addition lines. Flow sorted chromosomes were identified by fluorescence in situ hybridization. PCR with chromosome-specific primers confirmed the identity of sorted fractions and indicated suitability of sorted chromosomes for physical mapping. The possibility to sort large numbers of chromosomes opens a way for the construction of large-insert chromosome-specific DNA libraries in rye.
Trvalý link: http://hdl.handle.net/11104/0069471