0172418 - UEB-Q 20033145 RIV SIGLE FR eng C - Konferenční příspěvek (zahraniční konf.)
Vaňková, Radomíra - Kuncová, Gabriela - Podrazký, Ondřej - Gaudinová, Alena - Vaněk, Tomáš
Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy.
Proceedings. France: Bioencapsulation Research Group, 2003, s. 1-4.
[International Workshop on Bioencapsulation /11./. Illkirch (FR), 25.03.2003-27.03.2003]
Grant CEP: GA MŠMT OC 840.20
Výzkumný záměr: CEZ:AV0Z4072921; CEZ:AV0Z5038910
Klíčová slova: fluorescence spectroscopy * immobilization * dimethyl sulfoxide
Kód oboru RIV: CE - Biochemie

Using two-dimensional fluorescence spectroscopy, the growth of tobacco cells immobilized in alginate and pectate was monitored during the cultivation of the beads via the fluorescence of proteins (especially of tryptophan). Cell viability was followed by determination of their esterase activity using fluorescein diacetate as a substrate. This technique enables to measure the fluorescence of two fluorophores simultaneously. The effect of several stresses, (namely high and low temperature and the presence of organic solvent dimethylsulfoxide) was followed. Suitability of this technique to monitor the metabolic activities of cells within the beads was demonstrated.
Trvalý link: http://hdl.handle.net/11104/0069457