0172335 - UEB-Q 20033060 RIV CZ eng J - Článek v odborném periodiku
Šindelářová, Milada - Šindelář, Luděk
Influence of Antiviral Factor on Tobacco Mosaic Virus RNA Biosynthesis in Tobacco.
Biologia Plantarum. Roč. 46, č. 1 (2003), s. 95-100. ISSN 0006-3134. E-ISSN 1573-8264
Grant CEP: GA ČR GA522/99/1264
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Nicotiana tabacum * antiviral factor * TMV
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 0.919, rok: 2003

An antiviral factor (AVF) was separated by removing virus particles from extracts of tobacco mosaic virus (TMV) infected leaves using calcium phosphate gel and by column chromatography on DEAE cellulose. AVF was not found in the extracts from healthy plants. The AVF restricted the virus infectivity "in vivo" and significantly decreased the activity of key enzymes of metabolic pathways tending to the purine and pyrimidine nucleotides biosynthesis of viral-RNA (glucose-6-phosphate dehydrogenase, ribonucleases, phosphomonoesterase and phosphodiesterase). No inhibition of these enzymes was observed "in vitro" when the effect of different concentrations of AVF (0.25 - 250 mug cm(-3)) was examined.
Trvalý link: http://hdl.handle.net/11104/0069374