0172264 - UEB-Q 20023049 RIV US eng J - Článek v odborném periodiku
Link, V. - Hofmann, M. - Sinha, A. - Ehness, R. - Strnad, Miroslav - Roitsch, T.
Biochemical evidence for the activation of distinct subsets of mitogen-activated protein kinases by voltage and defense-related stimuli.
Plant Physiology. Roč. 128, č. 1 (2002), s. 271-281. ISSN 0032-0889. E-ISSN 1532-2548
Grant CEP: GA MŠMT OC 844.10
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: TOBACCO SUSPENSION CULTURE * CYCLIN DEPENDENT KINASES * CYTOSOLIC CALCIUM ION
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.800, rok: 2002

Activation of mitogen-activated protein (MAP) kinases is a common reaction of plant cells in defense-related signal transduction pathways. To gain insight into the mechanisms that determine specificity in response to a particular stimulus, a biochemical approach has been employed. Photoautotrophic suspension culture cells of tomato (Lycopersicon peruvianum) were used as experimental system to characterize MAP kinase activation by different stress-related stimuli. An elicitor preparation of the tomato-specific pathogen Fusarium oxysporum lycopersici was shown to result in the simultaneous induction of four kinase activities that could be separated by ion-exchange chromatography. The simultaneous activation of multiple MAP kinases was further substantiated by distinct pharmacological and immunological properties: a differential sensitivity toward various protein kinase inhibitors and a differential cross-reaction with isoform-specific MAP kinase antibodies. In contrast to the two fungal elicitors chitosan and the F. oxysporum lycopersici preparation, the plant-derived stimuli polygalacturonic acid and salicylic acid were shown to activate distinctly different subsets of MAP kinases.
Trvalý link: http://hdl.handle.net/11104/0069303