Aromatic cytokinins in micropropagated potato plants

0172256 - UEB-Q 20023041 RIV FR eng J - Článek v odborném periodiku
Baroja, F. E. - Aguirreolea, J. - Martínková, Hana - Hanuš, Jan - Strnad, Miroslav
Aromatic cytokinins in micropropagated potato plants.
Plant Physiology and Biochemistry. Roč. 40, č. 3 (2002), s. 217-224. ISSN 0981-9428
Grant CEP: GA MŠMT OC 844.10; GA ČR GA301/02/0475
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Acclimatization * Aromatic cytokinins * Micropropagation
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.582, rok: 2002

Endogenous cytokinins were studied in three micropropagated Solanum tuberosum L. cultivars (Kennebec, Turia and Jaerla) differing in survival after transplanting. Leaf and stem cytokinins were determined both in vitro and 10 d after being transferred to ex vitro conditions by a combination of high-performance liquid chromatography and enzyme-linked immunosorbent assay. Nine aromatic and nine isoprenoid type cytokinins were identified. Higher levels of total cytokinins mainly aromatics (92%) were detected in Kennebec, the cultivar showing better in vitro growth and 99% survival. On the contrary, a predominance of isoprenoid cytokinins (up to 57%) was observed after transplanting in Jaerla, the cultivar showing lower viability. Significant survival improvement was obtained in the Jaerla cultivar after addition to the culture medium of the aromatic cytokinin meta-topolin riboside (mTR). We also report here isolation and identification of this cytokinin by several sophisticated techniques including mTR-specific immunoaffinity chromatography, diode-array high-performance liquid chromatography (HPLC), and gas chromatographyůmass spectrometry of permethylated HPLC fractions. The occurrence of the aromatic cytokinins in potato plants is described for the first time.
Trvalý link: http://hdl.handle.net/11104/0069295