0172222 - UEB-Q 20023004 RIV CZ eng J - Journal Article
Hofman, Petr - Haisel, Daniel - Komenda, J. - Vágner, Martin - Tichá, I. - Schäfer, C. - Čapková, Věra
Impact of in vitro cultivation conditions on stress responses and on changes in thylakoid membrane proteins and pigments of tobacco during ex vitro acclimation.
Biologia Plantarum. Roč. 45, č. 2 (2002), s. 189-195. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA MŠMT OC 843.50; GA AV ČR IAA5038207
Institutional research plan: CEZ:AV0Z5038910
Keywords : thylakoid * proteins * tobacco
Subject RIV: EF - Botanics
Impact factor: 0.583, year: 2002

Four physiologically and phenotypically diversified tobacco (Nicotiana tabacum L. cv. Samsun) plantlet variants had been generated by cultivation on media either lacking or containing sucrose (0 and 3 %, m/v) under two different photon flux densities (PFD), 50 umol m"2 s"1 (LL) and 200 umol m"2 s'1 (HL). Plantlets were transferred into soil without any pre-acclimation and grown either under PFD of 200 umol m"2 s"1 or 700 umol m"2 s"1. Sucrose feeding in vitro resulted in reduced degree and duration of wilting after transfer. The highest readiness for ex vitro acclimation was found in 3 % HL plants, in which changes of photosynthetic apparatus and stress responses were the smallest. On the contrary, the steepest decline of Fv/Fm ratio on the first day after transplantation, doubled chlorophyll content and almost tripled Dl/LHC 2 ratio after 7 d of ex vitro growth under 700 umol m"2 s'1 characterized 0 % HL plants, which had suffered chronic photoinhibition in vitro.
Permanent Link: http://hdl.handle.net/11104/0069261