0162341 - UIACH-O 20010092 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Čáslavský, Josef - Chmelík, Josef
Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides.
Proceedings of the 18th Informal Meeting on Mass Spectrometry. Prague, 2000, s. 26. ISBN 80-238-5344-9.
[Informal meeting on mass spectrometry /18./. Praha (CZ), 30.04.2000-04.05.2000]
Grant ostatní: COPERNICUS(BE) ERB IC-15-CT98-0909
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: mass spectrometry * MALDI-TOF * oligosaccharides
Kód oboru RIV: CB - Analytická chemie, separace

MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples.
Trvalý link: http://hdl.handle.net/11104/0059659