Počet záznamů: 1  

Fucosylated glycans in the periventricular structures and the cerebrospinal fluid of the fetal rat forebrain. An autoradiographic and lectin binding histiotopic study

  1. 1.
    0142144 - FGU-C 20010200 RIV GB eng J - Článek v odborném periodiku
    Mareš, Vladislav - Brückner, G.
    Fucosylated glycans in the periventricular structures and the cerebrospinal fluid of the fetal rat forebrain. An autoradiographic and lectin binding histiotopic study.
    International Journal of Developmental Neuroscience. Roč. 19, č. 3 (2001), s. 297-303. ISSN 0736-5748
    Výzkumný záměr: CEZ:AV0Z5011922
    Klíčová slova: fetal rat brain * fucosylated glycans * cerebrospinal fluid
    Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
    Impakt faktor: 2.156, rok: 2001

    Autoradiographic 3H-fucose incorporation study of the brains of 20-day-old rat fetusses showed that the synthesis of fucosylated glycans is significantly higher in the ventricular germinative zone of the forebrain hemisphere than in the more superficial layers, including the cortical plate. Intense incorporation of 3H-fucose also occurred in the choroid plexus, both its epithelial and stromal component, in the primordial ependymal lining of the lateral ventricles, meninges and capillaries of the forebrain parenchyma. In the lateral ventricles, densely labeled microprecipitates of the cerebrospinal fluid (CSF) were occasionally observed. The histiotopic differences in 3H-fucose labeling were absent, or were much less expressed, in the autoradiograms prepared from unfixed cryostat sections containing mainly unincorporated isotope. This indicates that the blood-mediated supply of 3H-fucose to the studied brain compartments was essentially equal and our incorporation data reflect actual differences in the rate of fucosylation within the forebrain hemispheres. The cytochemical lectin-binding assay, carried out with Ulex europaeus and Lotus tetragonolobus agglutinins, showed that regions with a higher rate of 3H-fucose incorporation were also richer in fucose-bearing glycoconjugates. The study indicates that the periventricular regions and the cerebrospinal fluid of fetal rat forebrain form a fucosylated glycan-enriched chemoarchitectonic complex which may be of importance for control of some developmental processes, including maintaining the germinative activity of the primary neuropetihelium and growth of the telencephalic hemispeheres.
    Trvalý link: http://hdl.handle.net/11104/0039853