0104358 - UEB-Q 20043022 RIV NL eng J - Článek v odborném periodiku
Doležel, Jaroslav - Carter, N. - Ferguson-Smith, M. A.
Chromosomes go with the flow.
[Třídění chromozomů.]
Chromosome Research. Roč. 12, č. 1 (2004), s. 1-4. ISSN 0967-3849. E-ISSN 1573-6849
Grant CEP: GA ČR GA521/04/0607; GA ČR GA521/03/0595
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: flow cytometry * chromosomes
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.346, rok: 2004

Flow cytometry involves the analysis of fluorescence and light scatter properties of microscopic particles at high speed. The method was originally developed for the analysis of blood cells, and up to now, most flow cytometers are being used to evaluate human cells stained with various dyes and labelled with a variety of antibodies. The range of applications has continued to increase and encompass the analysis of ploidy, cell cycle kinetics and presence of specific antigens. The meeting of flow cytometry and cytogenetics gave rise to a completely new area of research. Flow cytogenetics, as the application of flow cytometry for analysis and sorting of mitotic chromosomes is usually termed, has contributed significantly to the progress in many areas of genome analysis and mapping as well as underpinning the sequencing of the human genome. The purpose of this article is to review the current applications of flow cytogenetics and its future potential.

Průtoková cytometrie je vynikající technologie, která byla původně vynalezena pro analýzu krevních buněk. Spektrum aplikací se postupně rozšířilo na měření ploidie, kinetiky buněčného cyklu a výskyt specifických antigenů. Zajímavou aplikací je třídění mitotických chromozomů.
Trvalý link: http://hdl.handle.net/11104/0011632