Počet záznamů: 1

Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography–tandem mass spektrometry

  1. 1.
    0369497 - FGU-C 2012 RIV NL eng J - Článek v odborném periodiku
    Syslová, K. - Rambousek, Lukáš - Kuzma, Marek - Najmanová, V. - Bubeníková-Valešová, V. - Šlamberová, R. - Kačer, P.
    Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography–tandem mass spektrometry.
    Journal of Chromatography A. Roč. 1218, č. 21 (2011), s. 3382-3391 ISSN 0021-9673.
    [International Symposium on Capillary Chromatography /34./ and the GCxGC Symposium /7./. Riva del Garda, 30.05.2011-04.06.2011]
    Grant ostatní: GA ČR(CZ) GA305/09/0126; GA ČR(CZ) GAP303/10/0580; GA ČR(CZ) GD203/08/H032
    Výzkumný záměr: CEZ:AV0Z50110509; CEZ:AV0Z50200510
    Klíčová slova: HPLC-MS * mass spectroscopy * microdialysis * dopamine
    Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
    Impakt faktor: 4.531, rok: 2011

    A sensitive assay method was developed for a parallel, rapid and precise determination of dopamine and its metabolites, homovanillic acid, 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid, from brain microdialysates. The method consisted of a pre-treatment step, freeze-drying (lyophilization), to concentrate dopamine and its metabolites from the microdialysates, and a detection step using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)
    Trvalý link: http://hdl.handle.net/11104/0203546