Počet záznamů: 1

Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy

  1. 1.
    0369489 - FGU-C 2012 RIV US eng J - Článek v odborném periodiku
    Michálek, Jan - Čapek, Martin - Kubínová, Lucie
    Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy.
    Microscopy Research Technique. Roč. 74, č. 9 (2011), s. 831-838 ISSN 1059-910X
    Grant CEP: GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733; GA MŠk(CZ) LC06063; GA MŠk(CZ) ME09010
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: confocal laser scanning microscopy * image enhancement * morphology filters
    Kód oboru RIV: JC - Počítačový hardware a software
    Impakt faktor: 1.792, rok: 2011

    In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
    Trvalý link: http://hdl.handle.net/11104/0203541