Počet záznamů: 1

Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes

  1. 1.
    0369083 - UFCH-W 2012 RIV US eng J - Článek v odborném periodiku
    Šachl, Radek - Humpolíčková, Jana - Štefl, Martin - Johansson, L. B. A. - Hof, Martin
    Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes.
    Biophysical Journal. Roč. 101, č. 11 (2011), L60-L62 ISSN 0006-3495
    Grant CEP: GA ČR GAP208/10/1090; GA ČR GAP208/10/0376
    Výzkumný záměr: CEZ:AV0Z40400503
    Klíčová slova: Foerster resonance energy transfer * nanodomains * Monte Carlo Setup
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 3.653, rok: 2011

    Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so convenient for determining domain sizes <20 nm. Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated.
    Trvalý link: http://hdl.handle.net/11104/0203233