Počet záznamů: 1

Development of ovarian hyperstimulation syndrome: interrogation of key proteins and biological processes in human follicular fluid of women undergoing in vitro fertilization

  1. 1.
    0368287 - UZFG-Y 2012 RIV GB eng J - Článek v odborném periodiku
    Jarkovská, Karla - Skalníková, Helena - Halada, Petr - Hrabáková, Rita - Moos, J. - Rezábek, K. - Gadher, S. J. - Kovářová, Hana
    Development of ovarian hyperstimulation syndrome: interrogation of key proteins and biological processes in human follicular fluid of women undergoing in vitro fertilization.
    Molecular Human Reproduction. Roč. 17, č. 11 (2011), s. 679-692 ISSN 1360-9947
    Výzkumný záměr: CEZ:AV0Z50450515; CEZ:AV0Z50200510
    Klíčová slova: biomarkers * computer modelling * human follicular fluid
    Kód oboru RIV: FK - Gynekologie a porodnictví
    Impakt faktor: 3.852, rok: 2011

    Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication and potentially life-threatening condition resulting from excessive ovarian stimulation during assisted reproductive technologies. Our aim was to identify candidate proteins in follicular fluid (FF) using various proteomic approaches which may help to identify patients at risk of OHSS. We analysed the proteome alterations in FF from patients suffering from severe forms of OHSS (OHSS+) compared with a control group of women without or with only mild signs of OHSS (OHSS-). The 12 abundant proteins of FF were removed using an immunoaffinity system. Pools of remaining depleted proteins were applied to the two-dimensional (2D) electrophoresis and 2D liquid chromatography and proteins in differentially expressed protein spots/fractions were identified by mass spectrometry.
    Trvalý link: http://hdl.handle.net/11104/0202675