Počet záznamů: 1

Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method

  1. 1.
    0368275 - UZFG-Y 2012 RIV PL eng J - Článek v odborném periodiku
    Marounek, Milan - Bubancová, I. - Podsedníček, M. - Lukešová, D. - Ramadan, M. A.
    Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method.
    Journal of Animal and Feed Sciences. Roč. 20, č. 3 (2011), s. 472-479 ISSN 1230-1388
    Grant CEP: GA ČR GD525/08/H060
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: phosphorus * phytate * dephosphorylation
    Kód oboru RIV: GM - Potravinářství
    Impakt faktor: 0.636, rok: 2011

    Activity of phytate dephosphorylation was determined in 26 samples of cereals, legumes, oilseeds and complete diets for poultry using the colourimetric and isotachophoretic method. The former method was based on the colour formed upon the reaction of molybdate with phosphate released from phytate. The latter method was based on measurement of decrease of phytate concentration in the reaction mixture. Rye had by far the highest phytate dephosphorylation activity, followed by wheat and wheat-containing diets. However, for most of the other samples, this activity was low. Phytate dephosphorylation activity as measured by capillary isotachophoresis was numerically lower than activity measured using the molybdate method; however, activities assayed by both methods correlated with statistical significance (r=0.86; P<0.001). The isotachophoretic method may be suitable for phytate dephosphorylation activity assay in feeds with a high content of inorganic phosphate and in samples of digesta and faeces.
    Trvalý link: http://hdl.handle.net/11104/0202666