Počet záznamů: 1

Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

  1. 1.
    0368065 - UFCH-W 2012 RIV GB eng J - Článek v odborném periodiku
    Norris, S. C. P. - Humpolíčková, Jana - Amler, Evžen - Huranová, Martina - Buzgo, Matej - Macháň, Radek - Lukáš, D. - Hof, Martin
    Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds.
    Acta Biomaterialia. Roč. 7, č. 12 (2011), s. 4195-4203 ISSN 1742-7061
    Grant CEP: GA MŠk(CZ) LC06063; GA AV ČR IAA500390702; GA ČR GAP304/10/1307
    Výzkumný záměr: CEZ:AV0Z40400503; CEZ:AV0Z50390512; CEZ:AV0Z50390703; CEZ:AV0Z50520514
    Klíčová slova: fluorecence dynamics * transient binding * diffusion
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 4.865, rok: 2011

    Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules.
    Trvalý link: http://hdl.handle.net/11104/0006700