Počet záznamů: 1

Applications of phasor plots to in vitro protein studies

  1. 1.
    0367712 - UFCH-W 2012 RIV US eng J - Článek v odborném periodiku
    James, N. G. - Ross, J. A. - Štefl, Martin - Jameson, D. M.
    Applications of phasor plots to in vitro protein studies.
    Analytical Biochemistry. Roč. 410, č. 1 (2011), s. 70-76 ISSN 0003-2697
    Grant CEP: GA MŠk(CZ) LC06063
    Výzkumný záměr: CEZ:AV0Z40400503
    Klíčová slova: protein fluorescence * kinetics * lifetimes
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 2.996, rok: 2011

    In a recent article, we described the application of phasor analysis to fluorescence intensity decay data on in vitro samples. As detailed in that article, this method provides researchers with a simple graphical method for viewing lifetime data that can be used to quantify individual components of a mixture as well as to identify excited state reactions. In the current article, we extend the use of in vitro phasor analysis to intrinsic protein fluorescence. We show how alterations in the excited state properties of tryptophan residues are easily visualized using the phasor method. Specifically, we demonstrate that protein-ligand and protein-protein interactions can result in unique shifts in the location of phasor points, indicative of protein conformational changes. Application of the method to a rapid kinetic experiment is also shown.
    Trvalý link: http://hdl.handle.net/11104/0202290