Počet záznamů: 1

Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus)

  1. 1.
    0367388 - BC-A 2012 RIV NL eng J - Článek v odborném periodiku
    Oliveira, U. C. - Fraga, J. S. - Licois, D. - Pakandl, Michal - Gruber, A.
    Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus).
    Veterinary Parasitology. Roč. 176, 2/3 (2011), 275-280 ISSN 0304-4017
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Eimeria * Rabbit * Coccidiosis * ITS1 * Ribosomal DNA * PCR-based diagnosis
    Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
    Impakt faktor: 2.579, rok: 2011

    Coccidiosis are the major parasitic diseases in poultry and other domestic animals including the domestic rabbit (Oryctolagus cuniculus). 11 distinct Eimeria species have been identified in this host, but no PCR-based method has been developed so far for unequivocal species differentiation. In this work, we describe the development of molecular diagnostic assays that allow for the detection and discrimination of the 11 Eimeria species that infect rabbits. We determined the nucleotide sequences of the ITS1 ribosomal DNAs and designed species-specific primers for each species. We performed specificity tests using heterologous sets of primers and DNA samples, and no cross-specific bands were observed. We obtained a detection limit varying from 500 fg to 1 pg, which corresponds approximately to 0.8-1.7 sporulated oocysts, respectively. The test reported here showed good reproducibility and presented a consistent sensitivity with three different brands of amplification enzymes.
    Trvalý link: http://hdl.handle.net/11104/0202094