Počet záznamů: 1

Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses

  1. 1.
    0367362 - BC-A 2012 RIV IT eng J - Článek v odborném periodiku
    Fránová, Jana
    Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses.
    Bulletin of Insectology. Roč. 64, Suppl. (2011), s. 287-288 ISSN 1721-8861
    Grant CEP: GA MŠk OC09021; GA AV ČR(CZ) 1QS500510558; GA MZe QH71145
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: DNA extraction * PCR * primers * RFLP
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 0.592, rok: 2011

    Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported.
    Trvalý link: http://hdl.handle.net/11104/0202071