Počet záznamů: 1

Unlocking the Barley Genome by Chromosomal and Comparative Genomics

  1. 1.
    0365200 - UEB-Q 2012 RIV US eng J - Článek v odborném periodiku
    Mayer, K. F. X. - Martis, M. - Hedley, P. E. - Šimková, Hana - Liu, H. - Morris, J. A. - Steuernagel, B. - Taudien, S. - Kubaláková, Marie - Suchánková, Pavla - Doležel, Jaroslav - Stein, N.
    Unlocking the Barley Genome by Chromosomal and Comparative Genomics.
    Plant Cell. Roč. 23, č. 4 (2011), s. 1249-1263 ISSN 1040-4651
    Výzkumný záměr: CEZ:AV0Z50380511
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 8.987, rok: 2011

    We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign -86% of the estimated -32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
    Trvalý link: http://hdl.handle.net/11104/0200497
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