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PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis

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    0365069 - UMG-J 2012 RIV US eng J - Článek v odborném periodiku
    Hrdinka, Matouš - Dráber, Peter - Štěpánek, Ondřej - Ormsby, Tereza - Otáhal, Pavel - Angelisová, Pavla - Brdička, Tomáš - Pačes, Jan - Hořejší, Václav - Drbal, Karel
    PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis.
    Journal of Biological Chemistry. Roč. 286, č. 22 (2011), s. 19617-19629 ISSN 0021-9258
    Grant CEP: GA MŠk 1M0506
    Grant ostatní: GAČR(CZ) MEM/09/E011
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: PRR7 * transmembrane adaptor protein * apoptosis
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.773, rok: 2011

    A highly conserved ransmembrane adaptor protein (TRAP) PRR7 was found to be readily up-regulated in activated human peripheral blood lymphocytes. Transient overexpression of PRR7 in Jurkat T cell line led to gradual apoptotic death dependent on the WW domain binding motif surrounding Tyr-166 in the intracellular part of PRR7. Jurkat clones with inducible expression of PRR7 (J-iPRR7) were generated. In these cells acute induction of PRR7 expression had a dual effect. It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Expression of PRR7 inhibited general tyrosine phosphorylation and calcium influx after T cell receptor cross-linking by antibodies. PRR7 was constitutively tyrosine-phosphorylated and associated with Src. PRR7 is a potential regulator of signaling and apoptosis in activated T cells.
    Trvalý link: http://hdl.handle.net/11104/0200405
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