Počet záznamů: 1

Primer Sequence Disclosure: A Clarification of the MIQE Guidelines

  1. 1.
    0364331 - BTO-N 2012 RIV US eng J - Článek v odborném periodiku
    Bustin, S.A. - Benes, V. - Garson, J.A. - Hellemans, J. - Huggett, J. - Kubista, Mikael - Mueller, R. - Nolan, T. - Pfaffl, M.W. - Shipley, G.L. - Vandesompele, J. - Wittwer, C.T.
    Primer Sequence Disclosure: A Clarification of the MIQE Guidelines.
    Clinical Chemistry. Roč. 57, č. 6 (2011), s. 919-921 ISSN 0009-9147
    Výzkumný záměr: CEZ:AV0Z50520701
    Klíčová slova: real-time PCR * MIQE * quality
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 7.905, rok: 2011

    The MIQE guidelines has rapidly become standard reporting procedure when writing up and presenting real-time quantitative pCR (qPCR) data. Accoeding to the originál requirement of MIQE full disclosure of primer sequence information is requested. This requirement is not possible to meet when using commersial primers from some leading vendors, since they disclose to clients only limited amount of informatik about the primers they supply. Recently they increased the information provided to clients to make experiments easier to design and easier to reproduce. In this letter the MIQE guidelines are amended to allow also these primers to be used in MIQE complient reports and publications.
    Trvalý link: http://hdl.handle.net/11104/0199843