Počet záznamů: 1

Single-cell gene-expression profiling and its potential diagnostic applications

  1. 1.
    0364328 - BTO-N 2012 RIV GB eng J - Článek v odborném periodiku
    Stahlberg, A. - Kubista, Mikael - Aman, P.
    Single-cell gene-expression profiling and its potential diagnostic applications.
    Expert Review of Molecular Diagnostics. Roč. 11, č. 7 (2011), s. 735-740 ISSN 1473-7159
    Grant CEP: GA ČR(CZ) GAP303/10/1338; GA ČR(CZ) GA301/09/1752
    Výzkumný záměr: CEZ:AV0Z50520701
    Klíčová slova: gene-expression profiling * RT-qPCR * single-cell gene-expression profiling
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.859, rok: 2011

    Gene-expression profiling has been successfully applied in various diagnostic applications, but its full capacity is yet to be realized. Samples are generally prepared from a mixture of different cells that are present in unknown proportions. Cells are, in many aspects, unique in their characteristics and this heterogeneity confounds the expression profile. The development of new and robust techniques to measure gene expression in single cells opens new avenues in molecular medicine. Today, gene-expression profiles of individual cells can be measured with high precision and accuracy, identifying different cell types as well as revealing heterogeneity among cells of the same kind. Here, we review practical aspects of single-cell gene-expression profiling using reverse transcription quantitative real-time PCR and its potential use in diagnostics
    Trvalý link: http://hdl.handle.net/11104/0006548