Počet záznamů: 1

A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae

  1. 1.
    0364285 - FGU-C 2012 RIV US eng J - Článek v odborném periodiku
    Barreto, L. - Canadell, D. - Petrezsélyová, Silvia - Navarrete, C. - Marešová, Lydie - Peréz-Valle, J. - Herrera, R. - Olier, I. - Giraldo, J. - Sychrová, Hana - Yenush, L. - Ramos, J. - Ariňo, J.
    A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae.
    Eukaryotic Cell. Roč. 10, č. 9 (2011), s. 1241-1250 ISSN 1535-9778
    Grant CEP: GA MŠk(CZ) LC531; GA AV ČR(CZ) IAA500110801
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: Potassium homeostasis * Saccharomyces cerevisiae * genomewide screen
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.604, rok: 2011

    Here, we describe a genome-wide screen for mutants that present altered tolerance to hygromycin B, spermine and tetramethylammonium. Two hundred twenty six mutant strains displayed altered tolerance to all three drugs (202 hypersensitive and 24 hypertolerant) and more than 50% presented a strong or moderate growth defect at limiting potassium (1 mM). Functional groups such as protein kinases and phosphatases, intracellular trafficking, transcription or cell cycle and DNA processing were enriched. Essentially, our screen has identified a substantial number of genes which were not previously described to play a direct or indirect role in potassium homeostasis. A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allow us to postulate the basis for the observed phenotypes
    Trvalý link: http://hdl.handle.net/11104/0199807