Počet záznamů: 1

Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases

  1. 1.
    0364215 - UOCHB-X 2012 RIV DE eng J - Článek v odborném periodiku
    Kielkowski, Pavel - Macíčková-Cahová, Hana - Pohl, Radek - Hocek, Michal
    Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases.
    Angewandte Chemie - International Edition. Roč. 50, č. 37 (2011), s. 8727-8730 ISSN 1433-7851
    Grant CEP: GA ČR GA203/09/0317
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: alkynes * DNA * protecting groups * nucleotides * restriction endonucleases
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 13.455, rok: 2011

    The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs.
    Trvalý link: http://hdl.handle.net/11104/0199751