Počet záznamů: 1

Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method

  1. 1.
    0361674 - UMCH-V 2012 RIV eng P - Patentový dokument
    Pytlík, R. - Hofman, P. - Trč, T. - Stehlík, D. - Soukup, T. - Kobylka, P. - Klener, P. - Rypáček, František - Mulinková, Katarína
    Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method.
    2011. Vlastník: Univerzita Karlova v Praze - Ústav hematologie a krevní transfuze - Ústav makromolekulární chemie AV ČR, v. v. i. Datum udělení patentu: 20.07.2011. Číslo patentu: EP1999250
    Grant CEP: GA MZd ND7448
    Výzkumný záměr: CEZ:AV0Z40500505
    Klíčová slova: mesenchymal stem cells * cultivation * bone-fracture treatment
    Kód oboru RIV: FJ - Chirurgie vč. transplantologie
    http://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20110720&DB=EPODOC&locale=en_EP&CC=EP&NR=1999250B1&KC=B1

    The invention relates to novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, the said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for about one to three weeks in CellGroTM Hematopoietic Stem cell Medium, certified for clinical use, with addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under standard conditions for the cultivation of tissue culture.
    Trvalý link: http://hdl.handle.net/11104/0198940