Počet záznamů: 1

Radiation damage to DNA in DNA-protein complexes

  1. 1.
    0361467 - UJF-V 2012 RIV NL eng J - Článek v odborném periodiku
    Spotheim Maurizot, M. - Davídková, Marie
    Radiation damage to DNA in DNA-protein complexes.
    Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. Roč. 711, 1-2 (2011), s. 41-48 ISSN 0027-5107
    Výzkumný záměr: CEZ:AV0Z10480505
    Klíčová slova: DNA-protein complex * ionizing radiation * molecular structure
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 2.850, rok: 2011

    The most aggressive product of water radiolysis, the hydroxyl (OH) radical, is responsible for the indirect effect of ionizing radiations on DNA in solution and aerobic conditions. Monte-Carlo based model RADACK, allows calculating the relative probability of damage of each nucleotide of DNA irradiated alone or in complexes with proteins. The confrontation of the calculated values with the results of the radiolytic footprinting experiments together with molecular modeling calculations show that: (1) the extent and location of the lesions are strongly dependent on the structure of DNA, which in turns is modulated by the base sequence and by the binding of proteins and (2) the regions in contact with the protein can be protected against the attack by the hydroxyl radicals via masking of the binding site and by scavenging of the radicals.
    Trvalý link: http://hdl.handle.net/11104/0198775