Počet záznamů: 1

Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions

  1. 1.
    0361375 - UOCHB-X 2012 RIV GB eng J - Článek v odborném periodiku
    Sorokin, V. A. - Valeev, V. A. - Usenko, E. L. - Andrushchenko, Valery
    Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions.
    International Journal of Biological Macromolecules. Roč. 48, č. 2 (2011), s. 369-374 ISSN 0141-8130
    Grant CEP: GA ČR GAP208/10/0559
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: DNA melting * alkaline solution * metal complex * differential UV spectroscopy * thermal denaturation
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 2.453, rok: 2011

    Effect of Mg2+, Ca2+, Ni2+ and Cd2+ ions on parameters of DNA helix-coil transition in sodium cacodylate (pH 6.5), Tris (pH 8.5) and sodium tetraborate (pH 9.0) buffers have been studied by differential UV-visible spectroscopy and thermal denaturation. Anomalous behavior of the melting temperature Tm and the melting interval .delta.Т in the presence of MgCl2 was observed in Tris, but not in cacodylate or tetraborate buffers. Decrease of the Tm and .delta.Т of DNA in the presence of Ni2+ and Cd2+ was caused by preferential ion interaction with N7 of guanine. This type of interaction was also found for Mg2+ in Tris buffer. The anomalous decrease in the Tm and .delta.Т values was connected to formation of complexes between metal ions and Tris molecules.
    Trvalý link: http://hdl.handle.net/11104/0198698