Počet záznamů: 1

Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases

  1. 1.
    0360993 - UOCHB-X 2012 RIV DE eng J - Článek v odborném periodiku
    Macíčková-Cahová, Hana - Pohl, Radek - Hocek, Michal
    Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases.
    Chembiochem. Roč. 12, č. 3 (2011), s. 431-438 ISSN 1439-4227
    Grant CEP: GA MŠk LC512; GA ČR GA203/09/0317
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: base-modified DNA * DNA cleavage * DNA polymerases * nucleosides * restriction endonucleases
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 3.944, rok: 2011

    A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved.
    Trvalý link: http://hdl.handle.net/11104/0198416