Počet záznamů: 1

Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase

  1. 1.
    0360800 - MBU-M 2012 RIV GB eng J - Článek v odborném periodiku
    Grulich, Michal - Maršálek, Jaroslav - Kyslík, Pavel - Štěpánek, Václav - Kotík, Michael
    Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase.
    Process Biochemistry. Roč. 46, č. 2 (2011), s. 526-532 ISSN 1359-5113
    Grant CEP: GA ČR GAP207/10/0135; GA ČR GAP504/10/0137
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Overexpression * Fed-batch * Aqueous two-phase system
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.627, rok: 2011

    Optimised culture conditions for the recombinant prototrophic strain Escherichia coli RE3(pSEKau2) were established to obtain biomass of epoxide hydrolase (EH). The recombinant enzyme was extracted from crude extracts using an optimised polyethylene glycol-based aqueous two-phase system (ATPS). Eupergit C 250L carrier proved to be the most interesting in terms of partially purified EH immobilization. The immobilized EH exhibited a significantly higher thermal stability compared to the free enzyme. Immobilization did not alter, to a large extent, the enantioselectivity and regioselectivity of the enzyme
    Trvalý link: http://hdl.handle.net/11104/0198266