Počet záznamů: 1

Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber

  1. 1.
    0360791 - MBU-M 2012 RIV JP eng J - Článek v odborném periodiku
    Siřišťová, L. - Luhový, R. - Sigler, Karel - Řezanka, Tomáš
    Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber.
    Extremophiles. Roč. 15, č. 3 (2011), 423-429 ISSN 1431-0651
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Thermophilic bacteria * Geobacillus * Meiothermus
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.941, rok: 2011

    Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains
    Trvalý link: http://hdl.handle.net/11104/0198258