Počet záznamů: 1

Genome mining for the discovery of new nitrilases in filamentous fungi

  1. 1.
    0360696 - MBU-M 2012 RIV NL eng J - Článek v odborném periodiku
    Kaplan, Ondřej - Bezouška, Karel - Malandra, Anna - Veselá, Alicja Barbara - Petříčková, Alena - Felsberg, Jürgen - Rinágelová, Anna - Křen, Vladimír - Martínková, Ludmila
    Genome mining for the discovery of new nitrilases in filamentous fungi.
    Biotechnology Letters. Roč. 33, č. 2 (2011), 309-312 ISSN 0141-5492
    Grant CEP: GA AV ČR IAA500200708; GA MŠk(CZ) LC06010; GA MŠk OC09046; GA MPO FT-TA5/043; GA ČR GD305/09/H008
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Aromatic nitrilase * Arylacetonitrilase * Aspergillus niger
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.683, rok: 2011

    Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate
    Trvalý link: http://hdl.handle.net/11104/0198185