Počet záznamů: 1

Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins

  1. 1.
    0359775 - UZFG-Y 2012 RIV GB eng J - Článek v odborném periodiku
    Procházka, Radek - Petlach, Michal - Nagyová, Eva - Němcová, Lucie
    Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins.
    Reproduction. Roč. 141, č. 4 (2011), s. 425-435 ISSN 1470-1626
    Grant CEP: GA ČR GA523/08/0111; GA MZe QI101A166
    Výzkumný záměr: CEZ:AV0Z50450515
    Klíčová slova: activatd protein kinase * EGF like factor * meiotic resumption
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.090, rok: 2011

    The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but notbetacellulin (BTC) in the cultured COCs. Both FSH and the EGF-like peptides significantly stimulated expression of expansion related genes (PTGS2, TNFAIP6 and HAS2) in the COCs and stimulated maturation of oocytes and expansion of cumulus cells. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. Significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or PMSG/hCG.
    Trvalý link: http://hdl.handle.net/11104/0197489