Počet záznamů: 1

DEAD-box RNA helicase is dispensable for mitochondrial translation in Trypanosoma brucei

  1. 1.
    0359433 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Richterová, Lenka - Vávrová, Zuzana - Lukeš, Julius
    DEAD-box RNA helicase is dispensable for mitochondrial translation in Trypanosoma brucei.
    Experimental Parasitology. Roč. 127, č. 1 (2011), 300-303 ISSN 0014-4894
    Grant CEP: GA ČR GA204/09/1667; GA MŠk LC07032; GA MŠk 2B06129
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Trypanosoma * Mitochondrial translation * RNA helicase * Cytochrome c oxidase * Mitochondrion
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.122, rok: 2011

    DEAD-box RNA helicase, a putative subunit of the mitochondrial ribosome of Trypanosoma brucei, has been down-regulated in the procyclic and bloodstream stage by RNA interference. Although ablation of the transcript leads to a week growth phenotype in the procyclic cells, the protein does not seem to be essential for mitochondrial translation under standard cultivation conditions, as shown by an assay that allows visualization of the de novo synthesized proteins. Furthermore, we show that synthesis of cytochrome c oxidase subunit I and cytochrome b does not occur in the mitochondrion of the bloodstream stage.
    Trvalý link: http://hdl.handle.net/11104/0197226