Počet záznamů: 1

Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi

  1. 1.
    0359409 - BC-A 2012 RIV NL eng J - Článek v odborném periodiku
    Paris, Zdeněk - Hashimi, Hassan - Lun, Sijia - Alfonzo, J. D. - Lukeš, Julius
    Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi.
    Molecular and Biochemical Parasitology. Roč. 176, č. 2 (2011), 116-120 ISSN 0166-6851
    Grant CEP: GA ČR GA204/09/1667; GA MŠk LC07032
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Trypanosoma * tRNA * Protein import * Mitochondrion * Kinetoplast
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.551, rok: 2011

    Trypanosoma brucei brucei has two distinct developmental stages, the procyclic stage in the insect and the bloodstream stage in the mammalian host. The significance of each developmental stage is punctuated by specific changes in metabolism. In the insect, T. b. brucei is strictly dependent on mitochondrial function and thus respiration to generate the bulk of its ATP, whereas in the mammalian host it relies heavily on glycolysis. Peculiarly, akinetoplastic strains of T. b. evansi that lack mitochondrial DNA do exist in the wild and are developmentally locked in the glycolysis-dependent bloodstream stage. Using RNAi we show that two mitochondrion-imported proteins, mitochondrial RNA polymerase and guide RNA associated protein 1, are still imported into the nucleic acids-lacking organelle of T. b. evansi, making the need for these proteins futile.
    Trvalý link: http://hdl.handle.net/11104/0197205