Počet záznamů: 1

MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

  1. 1.
    0359407 - BC-A 2012 RIV US eng J - Článek v odborném periodiku
    Ammerman, M. L. - Hashimi, Hassan - Novotná, Lucie - Číčová, Zdeňka - Mcevoy, S. M. - Lukeš, Julius - Read, L. K.
    MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process.
    R N A. Roč. 17, č. 5 (2011), 865-877 ISSN 1355-8382
    Grant CEP: GA ČR GA204/09/1667; GA MŠk 2B06129; GA MŠk LC07032
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: RNA editing * trypanosome * MRB1 complex * mitochondria * kinetoplast
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 5.095, rok: 2011

    In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.
    Trvalý link: http://hdl.handle.net/11104/0197204