Počet záznamů: 1

Localization of Double Bonds in Wax Esters by High-Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry Utilizing Fragmentation of Acetonitrile-Related Adducts

  1. 1.
    0359271 - UOCHB-X 2012 RIV US eng J - Článek v odborném periodiku
    Vrkoslav, Vladimír - Háková, Martina - Pecková, K. - Urbanová, Klára - Cvačka, Josef
    Localization of Double Bonds in Wax Esters by High-Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry Utilizing Fragmentation of Acetonitrile-Related Adducts.
    Analytical Chemistry. Roč. 83, č. 8 (2011), s. 2978-2986 ISSN 0003-2700
    Grant CEP: GA ČR GA203/09/0139
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: Ion-molecule reactions * lipids
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 5.856, rok: 2011

    Unsaturated wax esters (WEs) provided molecular adducts with C3H5N ([M+55]+.) in APCI sources in the presence of acetonitrile. CID of [M+55]+. gave fragments allowing localization of double bond(s) in hydrocarbon chains of WEs. These fragments were formed by cleavage of C-C bonds next to the double bond from its both sides. In methylene-interrupted polyunsaturated WEs, the diagnostic fragments related to each double bond were detected; the most abundant were those corresponding to cleavage of C-C bond next to the first and the last double bond. A simple nomenclature based on .alpha. and .omega. ions has been introduced. A data-dependent HPLC/APCI-MS2 method for comprehensive characterizing WEs in their complex mixtures has been developed and applied to natural mixtures of WEs isolated form jojoba oil and beeswax. More than 50 WE molecular species were completely identified, including information on acid and alcohol chain length and position of double bonds.
    Trvalý link: http://hdl.handle.net/11104/0197084