Počet záznamů: 1

Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol

  1. 1.
    0359057 - UCHP-M 2012 RIV CZ eng J - Článek v odborném periodiku
    Trögl, J. - Kuncová, Gabriela - Kuráň, P.
    Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol.
    Folia Microbiologica. Roč. 55, č. 6 (2010), s. 569-575 ISSN 0015-5632
    Grant CEP: GA MŠk ME 893
    Výzkumný záměr: CEZ:AV0Z40720504
    Klíčová slova: catabolic reporter bacterium * recombinant escheria-coli * optical biosensor
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 0.977, rok: 2010

    The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)(4). The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.
    Trvalý link: http://hdl.handle.net/11104/0196927