Počet záznamů: 1

Monte Carlo simulation of fluorescence correlation spectroscopy data

  1. 1.
    0358508 - UFCH-W 2012 RIV CZ eng J - Článek v odborném periodiku
    Košovan, P. - Uhlík, F. - Kuldová, J. - Štěpánek, M. - Limpouchová, Z. - Procházka, K. - Benda, Aleš - Humpolíčková, Jana - Hof, Martin
    Monte Carlo simulation of fluorescence correlation spectroscopy data.
    Collection of Czechoslovak Chemical Communications. Roč. 76, č. 3 (2011), s. 207-222 ISSN 0010-0765
    Grant CEP: GA AV ČR IAA400400621
    Výzkumný záměr: CEZ:AV0Z40400503
    Klíčová slova: Monte Carlo Study * fluorescence * spectroscopy
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 1.283, rok: 2011

    We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones.
    Trvalý link: http://hdl.handle.net/11104/0196531