Počet záznamů: 1

Complex I (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei

  1. 1.
    0358455 - BC-A 2012 RIV NL eng J - Článek v odborném periodiku
    Verner, Zdeněk - Čermáková, P. - Škodová, Ingrid - Kriegová, Eva - Horváth, A. - Lukeš, Julius
    Complex I (NADH:ubiquinone oxidoreductase) is active in but non-essential for procyclic Trypanosoma brucei.
    Molecular and Biochemical Parasitology. Roč. 175, č. 2 (2011), s. 196-200 ISSN 0166-6851
    Grant CEP: GA ČR GA204/09/1667; GA ČR GD206/09/H026; GA MŠk 2B06129; GA MŠk LC07032
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Trypanosoma * Mitochondrion * Respiration * Complex I
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.551, rok: 2011

    The requirement of complex I (NADH:ubiquionone oxidoreductase) for respiration in Trypanosoma brucei is controversial. Recent identification of its homologues in mitochondrial proteome resolved a question of its presence or absence. However, with one exception, no data have been available concerning the function(s) of complex I. Here we present a functional RNAi study of 3 (NUBM, NUKM, NUEM) putative subunits of this complex. Although no changes were detected in growth, mitochondrial membrane potential or reactive oxygen species production in cell lines depleted for target transcript, the NUBM and NUKM RNAi knock-downs showed decreased specific NADH:ubiquinone oxidoreductase activity. Moreover, glycerol gradients revealed the presence of two distinct peaks of NADH dehydrogenase activity, with shifted sensitivity to inhibitors of complex I upon RNAi induction. Thus complex I is not only present in the procyclic stage, but it does participate in electron transport chain.
    Trvalý link: http://hdl.handle.net/11104/0196495