Počet záznamů: 1

New model system for testing effects of flavonoids on doxorubicin-related formation of hydroxyl radicals

  1. 1.
    0358366 - UACH-T 2012 RIV US eng J - Článek v odborném periodiku
    Souček, P. - Kondrová, E. - Heřmánek, J. - Stopka, Pavel - Boumendjel, A. - Ueng, YF. - Gut, I.
    New model system for testing effects of flavonoids on doxorubicin-related formation of hydroxyl radicals.
    Anti-cancer Drugs. Roč. 22, č. 2 (2011), s. 176-184 ISSN 0959-4973
    Výzkumný záměr: CEZ:AV0Z40320502
    Klíčová slova: doxorubicin * electron spin resonance * flavonoids hydroxyl radicals
    Kód oboru RIV: FD - Onkologie a hematologie
    Impakt faktor: 2.407, rok: 2011

    Doxorubicin belongs to anthracycline cytotoxic drugs and it is widely used as a major therapeutic agent in the treatment of various types of tumors. However, its therapeutic use is limited by the development of myelosuppression and cardiotoxicity after a specific cumulative dose is reached. The aim of this study was to investigate the effect of flavonoids, either natural or synthetic on doxorubicin-mediated formation of oxidative stress implicated in doxorubicin toxicity. Doxorubicin caused a concentration-dependent increase in the formation of hydroxyl radicals in minipig liver microsomes used as an in-vitro model system. When bacterial membranes heterologously expressing human NADPH cytochrome-P450 oxidoreductase were incubated with doxorubicin, formation of the superoxide radical under aerobic conditions and the doxorubicin-semiquinone radical under anaerobic conditions was detected.
    Trvalý link: http://hdl.handle.net/11104/0196434