Počet záznamů: 1

LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients

  1. 1.
    0357968 - MBU-M 2011 RIV GB eng J - Článek v odborném periodiku
    Syslová, K. - Kačer, P. - Kuzma, Marek - Pankrácová, A. - Fenclová, Z. - Vlčková, Š. - Lebedová, J. - Pelclová, D.
    LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients.
    Journal of Breath Research. Roč. 4, č. 1 (2010), s. 1-8 ISSN 1752-7155
    Grant CEP: GA MZd NR9338
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: 8-ISOPROSTANE * ASBESTOS * MARKERS
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.828, rok: 2010

    The sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F2α a lipid oxidation biomarker, o-tyrosine an amino acid oxidation biomarker and 8-hydroxy-2_-deoxy-guanosine a nucleic acid oxidation biomarker. The method consisted of a pre-treatment part, freeze drtiny (lyophilization), serving the purpose of biomarkers concentration from the exhaled breath condensate and detection method LC-ESI-MS/MS, where the selected reaction-monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification
    Trvalý link: http://hdl.handle.net/11104/0196128