Počet záznamů: 1

Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)

  1. 1.
    0357655 - BU-J 2011 RIV US eng J - Článek v odborném periodiku
    Kvíderová, Jana
    Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta).
    Environmental Toxicology. Roč. 25, č. 6 (2010), s. 554-562 ISSN 1520-4081
    Grant CEP: GA MŠk 1M0571
    Výzkumný záměr: CEZ:AV0Z60050516
    Klíčová slova: bioassay * variable chlorophyll fluorescence * Chlorella kessleri
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 1.932, rok: 2010

    Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%).
    Trvalý link: http://hdl.handle.net/11104/0195870